Coding

Part:BBa_K5321017

Designed by: Yiyan Liao   Group: iGEM24_Peking   (2024-09-22)
Revision as of 14:35, 22 September 2024 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


MBP_SA_cPPVp

Maltose-binding protein (MBP) tag is a large (43 kDa) periplasmic and highly soluble protein of E. coli that acts as a solubility enhancer tag. It also increases protein expression levels. Considering of the large molecular weight of MBP, we were concerned that the MBP tag may interfere with the binding of SA and biotin. So we added a TEV-cutsite between the MBP and SA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 405
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1705
    Illegal AgeI site found at 1387
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 103


[edit]
Categories
Parameters
None