Coding

Part:BBa_K5321016

Designed by: Yiyan Liao   Group: iGEM24_Peking   (2024-09-22)
Revision as of 14:03, 22 September 2024 by Registry (Talk | contribs)

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MBP_SA_nPPVp

Maltose-binding protein (MBP) tag is a large (43 kDa) periplasmic and highly soluble protein of E. coli that acts as a solubility enhancer tag. It also increases protein expression levels. Considering of the large molecular weight of MBP, we were concerned that the MBP tag may interfere with the binding of SA and biotin. So we added a TEV-cutsite between the MBP and SA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2050
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2050
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 405
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2050
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2050
    Illegal NgoMIV site found at 1705
    Illegal AgeI site found at 1387
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None