Coding

Part:BBa_K5246011

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-22)
Revision as of 13:40, 22 September 2024 by Registry (Talk | contribs)

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CB2/CB2A HfsK Acetyltransferase

HfsK gene from Caulobacter crescentus encodes an acetyltransferase protein of 359aa. HfsK is c-di-GMP effector involved in holdfast biogenesis. Cells lacking HfsK form highly malleable holdfast structures with reduced adhesive strength that cannot support surface colonization. HfsK is a soluble protein but associates with the cell membrane during most of the cell cycle but is transfered to cytosol in the process of holdfast synthesis. HfsK deacetylates N-acetyl-glucosamine from the holfast which results in better adhesive properties.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 64
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 793
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None