Coding

Part:BBa_K5374014

Designed by: Langning Xu   Group: iGEM24_TestDaily-I-Beijing   (2024-09-17)
Revision as of 09:40, 17 September 2024 by Registry (Talk | contribs)

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AGR-EGFP (Agrin Domain). A multi-domain proteoglycan that binds collagen, fused with EGFP to determi

The Agrin domain plays a role in ECM organization and synapse formation, with collagen-binding capabilities. The fusion with EGFP allows for testing of its effect on protein activity, helping determine its suitability as a tag for controlled release systems in tissue engineering.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
    Illegal PstI site found at 826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 262
    Illegal NheI site found at 1309
    Illegal PstI site found at 352
    Illegal PstI site found at 826
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
    Illegal PstI site found at 826
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
    Illegal PstI site found at 826
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1952


[edit]
Categories
Parameters
None