Coding

Part:BBa_K5321011

Designed by: Yiyan Liao   Group: iGEM24_Peking   (2024-09-22)
Revision as of 05:22, 22 September 2024 by Registry (Talk | contribs)

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nPPVp_mut

To construct a molecular machine featuring a highly efficient sensor, such as an aptamer, and an upstream signal transporter, like a split protease, we have devised crosslinking via click chemistry, specifically DBCO-p-AzF click reaction, to do this task. We introduced UAG stop codon site-directed mutations into the nPPVp genes. This modification allows the pEvol-pAzFRS.2.t1 plasmids to incorporate p-AzF through an orthogonal tRNA synthetase/tRNA pair.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 295
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 295
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 295
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 295
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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