Composite
Part:BBa_K5036033
Designed by: Emad hamdy Matter Group: iGEM24_AFCM-Egypt (2024-09-21)
dCas9(C)_NLS-Syn-VEGFR-1 (VEGF-R1, N-TEV, NLS, TCS(Q,G),HA,dCas9(C),VP64,GFP)
In our first receptor chain, we've engineered a system that responds to tissue injury. An external domain, VEGF-R1, is attached to a protein sequence containing TEV protease, a nuclear localization signal (NES), a TEV cleavage site(TCS), and dCas9(C). When an injury occurs, VEGF levels rise outside the cell. This binds to VEGF-R1, activating the receptor and triggering the TEV protease to cleave the chain at the TCS site. This releases dCas9(C), which can then associate with a complementary dCas9 fragment (dCas9(N)) to form a fully functional dCas9 enzyme
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2173
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3958
Illegal NgoMIV site found at 4031
Illegal NgoMIV site found at 4516
Illegal NgoMIV site found at 5425 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 6722
Illegal SapI.rc site found at 3274
[edit]
Categories
Parameters
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