Composite

Part:BBa_K5036028

Designed by: Emad hamdy Matter   Group: iGEM24_AFCM-Egypt   (2024-09-21)
Revision as of 09:52, 21 September 2024 by Registry (Talk | contribs)

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dCas9(N)_NES-Syn-VEGFR-2 (VEGF-R2, N-TEV, NES, TCS (Q, L), HA, dCas9(N),mCherry)

In our second receptor chain, we've engineered a system that responds to tissue injury. An external domain, VEGF-R2, is attached to a protein sequence containing TEV protease, a nuclear export signal (NES), a TEV cleavage site(TCS), and dCas9(N). When an injury occurs, VEGF levels rise outside the cell. This binds to VEGF-R2, activating the receptor and triggering the TEV protease to cleave the chain at the TCS site. This releases dCas9(N), which can then associate with a complementary dCas9 fragment (dCas9(C)) to form a fully functional dCas9 enzyme


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 982
    Illegal BglII site found at 2341
    Illegal BglII site found at 3647
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4474
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1854
    Illegal BsaI.rc site found at 664
    Illegal BsaI.rc site found at 1442
    Illegal BsaI.rc site found at 2731
    Illegal SapI.rc site found at 3289


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Categories
Parameters
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