Coding

Part:BBa_K5246017

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-21)
Revision as of 15:48, 21 September 2024 by Registry (Talk | contribs)

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HB HfsE glycosyltransferase

The HfsE gene encodes a 518 amino acid protein in Hirschia baltica, identified as a putative integral membrane glycotransferase. The primary role of this protein is to transfer a glucose-1-phosphate from uridine diphosphate glucose (UDP-glucose) or to an undecaprenyl phosphate (UP), which is a crucial lipid carrier that facilitates the transfer of cell wall intermediates across the cytoplasmic membrane in bacteria. This reaction represents the first step in the synthesis of the holdfast polysaccharide repeat units in the cytoplasm. [1][2]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 48
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 214
    Illegal AgeI site found at 1381
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None