Coding

Part:BBa_K5321006

Designed by: Yiyan Liao   Group: iGEM24_Peking   (2024-09-21)
Revision as of 11:41, 21 September 2024 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Plum pox virus protease (PPVp), codon optimized for E. coli, 6x His tagged

Plum pox virus protease (PPVp), also referred to as the nuclear inclusion a protein (NIa), is a key enzyme from the plum pox virus (PPV). It plays a critical role as one of the three viral proteases involved in the maturation of the viral polyprotein into its functional components. Here, we used it as a tool to amplify our detection signal of disease biomarkers.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 712
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 712
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 712
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None