Part:BBa_K283023:Design

plux-rbs-CI-terminator-plambda(R-O12)-rbs-cheZ-terminator

The gene expression cheZ is controled by the cI regulated promoter K094002 which is based on the pR promoter from bacteriophage lambda. The promoter has two DNA binding sites for lambda cI repressor. cI binding results in repression of transcription. Our group intended to use this device to realize speed control system.

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 307
  Illegal EcoRI site found at 1792
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 307
  Illegal EcoRI site found at 1792
  Illegal NheI site found at 2472
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 307
  Illegal EcoRI site found at 1792
  Illegal BglII site found at 332
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 307
  Illegal EcoRI site found at 1792
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 307
  Illegal EcoRI site found at 1792
  
• 1000
  COMPATIBLE WITH RFC[1000]