Coding
Part:BBa_K5136058
Designed by: Xiaoxiao Zhang Group: iGEM24_XMU-China (2024-09-14)
rhlA
Required for rhamnolipid surfactant production.
Biology
The RhlA comes from Pseudomonas aeruginosa and can compete with the enzymes of the type II fatty acid synthase (FASII) cycle for the β-hydroxyacyl-acyl carrier protein (ACP) pathway intermediates, therefore supplies the acyl moieties for rhamnolipid biosynthesis. RhlA has a greater affinity for 10-carbon substrates, and is the only enzyme required to generate the lipid component of rhamnolipid. (1)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 69
Illegal XhoI site found at 805 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 294
Illegal BsaI.rc site found at 478
[edit]
Categories
Parameters
None |