Generator

Part:BBa_K274110

Designed by: Siming Ma   Group: iGEM09_Cambridge   (2009-09-18)
Revision as of 02:29, 25 September 2009 by Msmsimon (Talk | contribs)

CrtEBI under constitutive promoter

This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under constitutive promoter R0011.

Enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 converts colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene).

Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).

Datasheet for Part BBa_K274100 and Part BBa_274110 in E. coli strain MG1655. You may also wish to refer to the "Experience" page.

Lycopene datasheet.JPG

For PDF version of this Datasheet: File:Lycopene.pdf

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2037
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1573
    Illegal NgoMIV site found at 1703
    Illegal AgeI site found at 788
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis
//function/reporter/pigment
Parameters
None