Part:BBa_K5143020

AGA2, a pre-peptide that is secreted by the Saccharomyces cerevisiae yeast

ScCBD_cex'V1 : a Cellulose Binding Domain optimised for Saccharomyces cerevisiae, that enables the fixation of proteins to Bacterial Cellulose

Description

ScCBD_cex'V1 (Cellulose Binding Domain) is a peptide that has a huge affinity for bacterial cellulose. When fused with another protein, ScCBD_cex'V1 enables the fixation of the protein on the bacterial cellulose.

Figure 1: ScCBD_Cex'V1 three-dimensional structure

Figure 2: Functional cellulose

Figure 3: ScCBD_Cex coding sequence

Construction

ScCBD-Cex'V1 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a yellow chromoprotein (fwYellow) in order to color the bacterial cellulose in yellow: BBa_K5143023

References

Cappellaro, C. et al. Saccharomyces cerevisiae a- and alpha-agglutinin: characterization of their molecular interaction. The EMBO Journal 10, 4081–4088 (1991)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 256
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 226
  Illegal AgeI site found at 265
  
• 1000
  COMPATIBLE WITH RFC[1000]