Composite

Part:BBa_K243028

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-19)
Revision as of 22:54, 20 October 2009 by Sarah (Talk | contribs)

pMA FOS-Split Linker-Fok_a

Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. Nine homologues of the AP-1 leucine zipper region have been characterized. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July).

1fos bio r 250.jpgHeterodimeric Fos complex (http://www.rcsb.org/pdb/explore/images.do?structureId=1FOS)

Usage and Biology

Split Linker-Fok_a can be fused with Fos. The mixture of this construct with an other hybrid protein GST-FosW-His (simultanously expressed) allows the dimerization of Fos and therefore its binding at their target sequence TRE (TGACTCA) or CRE (TGACGTCA).

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Categories
Parameters
n/apMA FOS-Split Linker-Fok_a