Composite

Part:BBa_K5317008:Design

Designed by: Jan Gelhoet   Group: iGEM24_Hannover   (2024-09-13)
Revision as of 12:30, 13 September 2024 by Registry (Talk | contribs)

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MREwt promoter-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was important to consider the MRE site positioning for optimal MTF-1 and MRE site interaction and do not diturb the start and stop codon as well as avoid frame shifts of the EGFP sequence during cloning.


Source

The MRE wt promoter was synthesized and the EGFP gene was available from the EGFP-C2 backbone (K3338020)

References