Part:BBa_K237002
Constitutive RIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp.
RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Qourum sensing(QS) abilities of Staphylococcus species [1]. RIP effectively works as an antibiotic in its inhibition of bacterial biofilm-formation. It is important to note that even though it has the effect of an antibiotic [1] on bacterial load, the mechanism is a novel one, and doesn't appear to induce bacterial resistance [1].
RIP has the sequence YSPXTNF and has been tested extensively in a synthetic (as in chemical synthesis) form for ability to inhibit bacterial infections in mice [1] and biofilm formation on human cells [2].
Usage and Biology
This generator is under constitutive expression by a TetR repressible promoter BBa_R0040.
References
[1] Suppression of Drug-Resistant Staphylococcal Infections by the Quorum-Sensing Inhibitor RNAIII-Inhibiting Peptide. Journal of Infectious Diseases [serial on the Internet]. (2004, July 15), [cited October 21, 2009]; 190(2): 318-320. Available from: Academic Search Premier. [http://www.ncbi.nlm.nih.gov/pubmed/15216467]
[2] Prevention of Staphylococcus aureus biofilm on dialysis catheters and adherence to human cells. Balaban N, Gov Y, Bitler A, Boelaert JR. Kidney Int 2003; 63:340–5. [http://ncbi.nlm.nih.gov/pubmed/12472801]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |