Protein_Domain

Part:BBa_K283036

Designed by: Shi Lei   Group: iGEM09_HKU-HKBU   (2009-10-16)
Revision as of 17:17, 20 October 2009 by NelsonChu (Talk | contribs)

invasin(1-625aa)

The invasin protein of Yersinia pseudotuberculosis has been utilized for heterologous surface display. Y. pseudotuberculosis invasin promotes bacterial entry by binding to host cell integrins. It is a 986-residue protein of which the 500 N-terminal aa are thought to reside in the outer membrane. The C-terminal 497 residues, which make up the extracellular part, are involved in the binding to integrin. Nakajima and co-workers developed a system for expression of random peptides on the cell surface of E. coli by creation of a fusion hybrid between a peptide and the invasin protein. This part can be used to display fusion protein on the surface of bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 886
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/membrane
//proteindomain/localization
Parameters
None