Device

Part:BBa_K283000

Designed by: YU Bin, Qiang Qin Qin   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 08:52, 21 October 2009 by HKUHKBU (Talk | contribs)

pTet-rbs-lpp_ompA_eGFP_streptavidin-double Terminators

Streptavidin protein binds strongly to the biotin molecule, and is widely used to bind biotinylated materials. This device was designed for polar expression of streptavidin on the outer membrane of bacterial cells. To facilitate the observation, the fusion protein of Lpp signal peptide, OmpA aa46-66, and streptavidin wild-type (BBa_J36848) was fused with GFP-coding sequence (BBa_e0040). The expression will display streptavidin-GFP fusion protein on the cell surface. It has been demonstrated work as expected in our system (see experience or visit our wiki).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1314
    Illegal AgeI site found at 1365
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/generator/prct
Parameters
n/apTet-rbs-lpp_ompA_streptavidin-double Terminators