Coding
Part:BBa_K5036007
Designed by: Emad hamdy Matter Group: iGEM24_AFCM-Egypt (2024-09-12)
N-TEV
This is known as the tobacco etch virus which is very selective for cleaving proteins at particular amino acid sequences and has been modified to have better qualities like greater heat stability, decreased autolysis (self-cleavage) and also allows researchers to precisely cleave the tag off, leaving behind the receptor in its unmodified form. In our model, TEV was divided into N-terminal and C-terminal fragments. So the N-terminal fragment was grafted onto dCAS9(N) to generate TEV(N)-dCas9(N).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 316
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Categories
Parameters
None |