Plasmid

Part:BBa_K5101002

Designed by: Ziqi Mi   Group: iGEM24_ZQT-Nanjing   (2024-09-11)
Revision as of 08:27, 11 September 2024 by Registry (Talk | contribs)

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Plasmid pET29a-pJ23119-SoxR-T-pSoxS-RBS-eGFP-RBS-AMP-T7

This composite plasmid, pET29a-pJ23119-SoxR-T-pSoxS-RBS-eGFP-RBS-AMP-T7, is engineered for precise expression control in response to oxidative stress conditions. It incorporates a dual-promoter system where the first part consists of a strong constitutive promoter J23119 for consistent expression of eGFP, serving as a reporting system. Following the J23119, a nitric-oxide-inducible promoter system, SoxR/SoxS, is used to control the expression of an antimicrobial peptide (AMP), enabling specific activation under stress conditions. The plasmid is equipped with Ribosome Binding Sites (RBS) for optimal translation efficiency of both proteins, ensuring robust expression in E. coli Nissle 1917. The T7 terminator ensures transcriptional termination, maintaining system stability and efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 572
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1708
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1678
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None