Coding

Part:BBa_K180009

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-16)
Revision as of 14:11, 16 October 2009 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


mBanana (rights owned by Clontech)

mBanana fluorescent protein - Rights owned by Clontech. Sequence is commercially available from Clontech. Sequence obtained through PCR amplification, with BioBrick prefix and suffix added through in-house designed primers.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
    Illegal NgoMIV site found at 598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 541


[edit]
Categories
//cds/reporter/yfp
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
Parameters
colorYellow
directionforward
emission553 nm
excitation540 nm
n/aDsRed derivative
proteinmBanana