Part:BBa_K5396002

Nt2RepCt-SpyTag

Spidroins are the primary proteins that compose spider silk, renowned for their exceptional mechanical properties, including strength, elasticity, and biodegradability. These proteins are produced in specialized glands of spiders and it can have various functions, such as web construction, prey capture, and mobility.

NT2RepCT is a recombinant miniature spidroin protein that has been engineered to mimic the properties of natural spider silk proteins. It is composed of several key domains that enable it to self-assemble into hydrogels and fibers under the right conditions [ ].

This Nt2RepCt is fused with SpyCatcher protein from Streptococcus pyogenes that forms a covalent bond with a peptide called SpyTag, enabling stable assembly of multi-protein complexes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 895
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]