Coding

Part:BBa_K180001:Design

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-12)
Revision as of 10:42, 21 October 2009 by Rich (Talk | contribs) (→‎References)

Green fluorescent protein (+LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

LVA degradation tail and prefix/suffix addition by sequential PCR amplifications with specially designed primers.


Source

Based upon GFP from E0040, with LVA degradation tail attached.

References

A reference for the original part E0040 (GFPmut3b): Elowitz MB and Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature 2000 Jan 20; 403(6767) 335-8