Plasmid

Part:BBa_K5082010

Designed by: Fangyuan Duan   Group: iGEM24_YiYe-China   (2024-09-07)
Revision as of 10:45, 7 September 2024 by Registry (Talk | contribs)

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pMIR plasmid vector

1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 7332
    Illegal SpeI site found at 5344
    Illegal PstI site found at 2047
    Illegal PstI site found at 5601
    Illegal PstI site found at 7344
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7320
    Illegal SpeI site found at 5344
    Illegal PstI site found at 2047
    Illegal PstI site found at 5601
    Illegal PstI site found at 7344
    Illegal NotI site found at 92
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5090
    Illegal BamHI site found at 2792
    Illegal XhoI site found at 7326
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 7332
    Illegal SpeI site found at 5344
    Illegal PstI site found at 2047
    Illegal PstI site found at 5601
    Illegal PstI site found at 7344
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 7332
    Illegal SpeI site found at 5344
    Illegal PstI site found at 2047
    Illegal PstI site found at 5601
    Illegal PstI site found at 7344
    Illegal NgoMIV site found at 2498
    Illegal NgoMIV site found at 5708
    Illegal NgoMIV site found at 7052
    Illegal NgoMIV site found at 7073
    Illegal AgeI site found at 1822
    Illegal AgeI site found at 3926
    Illegal AgeI site found at 5209
    Illegal AgeI site found at 6776
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3752
    Illegal BsaI.rc site found at 1435
    Illegal SapI.rc site found at 2347
    Illegal SapI.rc site found at 2557
    Illegal SapI.rc site found at 6958


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Categories
Parameters
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