Reporter

Part:BBa_K5198012

Designed by: Robert Kaptur   Group: iGEM24_Duke   (2024-08-30)
Revision as of 15:49, 30 August 2024 by Registry (Talk | contribs)

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sfGFP (human codon optimized)

Human codon optimized sequence of superfolder Green Fluorescent Protein (sfGFP) [1]. The start codon is not included.

sfGFP is a mutated version of GFP (BBa_E0040) designed for stability and better folding. This variant of sfGFP can be used as a reporter of gene expression in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None