Coding

Part:BBa_K283005

Designed by: Yu bin, Jiang QinQin   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 08:04, 13 October 2009 by Registry (Talk | contribs)

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lpp_ompA_eGFP_streptavidin

This part consists of a fusion protein Lpp-rbs-protein under the pLac promoter. The Lpp is a signal peptide. The ompA domain of the fusion directs the protein to the surface membrane. The pLac promoter can be repressed by LacI protein. This part lacks a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1234
    Illegal AgeI site found at 1285
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/generator/prc
//function/reporter/fluorescence
Parameters
None