Coding

Part:BBa_K5117010

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-08-31)
Revision as of 11:52, 31 August 2024 by Jesa98 (Talk | contribs)


BhrPET

gene of the uncultured thermophilic bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase), a homologue of the leaf-branch compost cutinase (LCC), already codon optimized for Bacillus subtilis (Xi et al. 2021), but additionally including the signal peptide of the aprE gene for secretion in Bacillus subtilis

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
    Illegal AgeI site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None