Coding

Part:BBa_K5117010

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-08-31)
Revision as of 11:49, 31 August 2024 by Registry (Talk | contribs)

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BhrPET

gene of the uncultured thermophilic bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase), a homologue of the leaf-branch compost cutinase (LCC), already codon optimized for Bacillus subtilis (Xi et al. 2021), but additionally including a signal peptide SPaprE of the aprE gene for secretion in Bacillus subtilis

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
    Illegal AgeI site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None