Part:BBa_K5143002

Cp19k : a barnacle cement protein used as a bioglue, optimised for Saccharomyces cerevisiae

Description

Barnacle, a marine organism, easily stick to underwater matrices through the secretion of various proteins and forming cement complexes. The protein complex for barnacle, Cp19k plays a key role in interfacial adhesion.

Construction

The Cp19k gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae.

References

Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]