Coding

Part:BBa_K5226008

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-07-28)
Revision as of 14:31, 28 July 2024 by Registry (Talk | contribs)

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TD-sdaA

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. For the first method, based on previous studies obtained from literature research, we selected the tetrahydrofolate (THF) cycle imported from Methylobacterium extorquens AM1(parts:) and strengthened the endogenous C2 and C3 modules of Halomonas TD(parts:).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1119
    Illegal SpeI site found at 646
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 646
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1119
    Illegal SpeI site found at 646
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1119
    Illegal SpeI site found at 646
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None