Coding
MaSp1

Part:BBa_K5143001:Design

Designed by: Jess Philippon   Group: iGEM24_UnivLyon1-INSALyon   (2024-07-28)
Revision as of 12:06, 28 July 2024 by Registry (Talk | contribs)

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MaSp1 : a spider silk protein used as a bioglue, optimised for Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for DNA synthesis and for Saccharomyces cerevisiae.



Source

The source of the part will be its sequence which was retrieved from Yan et al., 2023.

References