Composite

Part:BBa_K4166013

Designed by: Matthew Hanson   Group: iGEM22_Alma   (2022-10-12)
Revision as of 14:27, 12 October 2023 by Matt 2001 (Talk | contribs)


DDT biosensor (RtER-TetR-RFP)

RFP under the control of Rainbow trout estrogen receptor by way of TetR: This is our biosensor for xenoestrogens like DDT. In the presence of such molecules, rtER will prevent expression of the TetR gene, which in turn relieves repression of RFP. In this case, TetR is an inverter module allowing for a red color signal in response to the xenoestrogen

Characterization of DDT Biosensor - Alma 2023

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1922
    Illegal NheI site found at 1945
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 908
    Illegal BamHI site found at 570
    Illegal XhoI site found at 464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3305
    Illegal AgeI site found at 3417
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None