Part:BBa_K4765122

ribozyme connected: pgmA + galU + mScarlet

Introduction

This composite part is designed for the production of exopolysaccharide(EPS), and detection of EPS’s adhesion ability. It includes E. coli pgmA on the downstream site and E. coli galU on the upstream site, two essential enzymes in EPS biosynthesis pathway. These two enzymes are tested by iGEM20_XJTU-China in BBa_K3331001(galU)and BBa_K3331002(pgmA). The following mScarlet is utilized to detect E. coli 's adhesion ability.

Usage and Biology

We use this part to improve EPS production of E. coli and assessment of EPS’s adhesion ability.

Characterization

Agarose gel electrophoresis

Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. From left lane(1) to right lane(3) indicate the successful construction of pgmA, pgmA + galU, and pgmA + galU + mScarlet.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 3285
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]