Composite

Part:BBa_K4805011

Designed by: Tianyin Zhao   Group: iGEM23_LINKS-China   (2023-10-12)
Revision as of 14:24, 12 October 2023 by Registry (Talk | contribs)

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pTEF2-ERG20_F96W-tSSA1-pPGK1-ClSS_ S532A,F441V-tPGK1

ERG20 catalyzes the sequential formation of geranyl diphosphate (GPP) and farnesyl diphosphate (FPP). ERG20_F96W, as a F96W mutant can help increase the supply of FPP. ClSS_S532A,F441V encodes for a variant of ClSS, a santalene synthase from Clausena lansium(Genbank Accession: ADR71055.1), which possesses two site direct mutagenesis of S532A and F441V. Our part can be a reference for other teams to perfect the pathway producing santalene from FPP. It belongs to the terpenoid part collection we have established for the production of santalol and ambrein in S. cerevisiae, which includes BBa_K4805000-BBa_K4805012.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1302
    Illegal BglII site found at 1363
    Illegal BglII site found at 2828
    Illegal BglII site found at 3917
    Illegal XhoI site found at 1479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1018
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1465
    Illegal BsaI site found at 1726
    Illegal BsaI.rc site found at 1715
    Illegal BsaI.rc site found at 2838


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