Coding

Part:BBa_K4805010

Designed by: Yuhan Gan   Group: iGEM23_LINKS-China   (2023-10-12)
Revision as of 14:16, 12 October 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pTEF2-ERG20_F96W-tSSA1-pPGK1-ClSS-tPGK1

ERG20 catalyzes the sequential formation of geranyl diphosphate (GPP) and farnesyl diphosphate (FPP). ERG20_F96W, as a F96W mutant can help increase the supply of FPP.

ClSS is a santalene synthase from Clausena lansium (Genbank Accession: ADR71055.1), which can catalyze the conversion of FPP(farnesyl diphosphate) into santalene. Compared with other santalene synthase, ClSS is capable of producing the most a-santalene(Jiachen Z. et al, 2022). In our project we expressed these two heterologous genes in the genome of S. cerevisiae in order to yield santalene.

Our part can inspire other teams to produce santalene from FPP. It belongs to the terpenoids part collection we have established for the production of santalol and ambrein in S. cerevisiae, which includes BBa_K4805000-BBa_K4805012.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1308
    Illegal BglII site found at 1369
    Illegal BglII site found at 2850
    Illegal BglII site found at 3945
    Illegal XhoI site found at 1493
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1024
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1479
    Illegal BsaI site found at 1748
    Illegal BsaI.rc site found at 1729
    Illegal BsaI.rc site found at 2860


[edit]
Categories
Parameters
None