![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Plasmid
Part:BBa_K5000000
Designed by: jian mo Group: iGEM23_Squirrel-Guangzhou (2023-10-11)
overexpression dxs, ispA, idi under the control of T7 promoter
This plasmid is used to enhance the production of isoprenoid precursor in E.coli by overexpression three key enzyme in the MEP pathway under the control of T7 promoter, the three genes are 1-deoxyxylulose-5-phosphate synthase (DXS), farnesyl diphosphatesynthase (IspA) and isopentenyl diphosphate isomerase (IDI) from E.coli.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 3483
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 953
Illegal SpeI site found at 3483 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 3489
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 3483
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 3483
Illegal AgeI site found at 249 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1758
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Categories
Parameters
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