Plasmid

Part:BBa_K5000000

Designed by: jian mo   Group: iGEM23_Squirrel-Guangzhou   (2023-10-11)
Revision as of 22:12, 11 October 2023 by Registry (Talk | contribs)

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overexpression dxs, ispA, idi under the control of T7 promoter

This plasmid is used to enhance the production of isoprenoid precursor in E.coli by overexpression three key enzyme in the MEP pathway under the control of T7 promoter, the three genes are 1-deoxyxylulose-5-phosphate synthase (DXS), farnesyl diphosphatesynthase (IspA) and isopentenyl diphosphate isomerase (IDI) from E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3483
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 953
    Illegal SpeI site found at 3483
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3489
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3483
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3483
    Illegal AgeI site found at 249
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1758


[edit]
Categories
Parameters
None