Regulatory

Part:BBa_K4825021

Designed by: Qiu Tianhou   Group: iGEM23_GreatBay-SCIE   (2023-10-12)
Revision as of 12:49, 12 October 2023 by Registry (Talk | contribs)

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pNOTAra1

pNOTAra1 is a synthetic repressible promoter designed for automation for yeast, in which the level of the co-repressor arabinose regulates the initiation of the transcription. AraC functions as a homodimer. In the absence of arabinose, the promoter is accessible. In the presence of arabinose, two subunits of AraC bind to adjacent Inv Aral1 and Inv Ara2 separated by four bp and the two subunits are connected through the dimerisation domain. In our project, this part is utilized to construct a plasmid with BAX gene and control its transcription by repressing it with the presence of arabinose in S.cerevisiae and prevent the initiation of kill switch.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None