Coding
GlrN

Part:BBa_K225000

Designed by: Nasa SAVORY   Group: iGEM09_Tokyo-Nokogen   (2009-10-15)
Revision as of 13:39, 20 October 2009 by Nasa (Talk | contribs)

Green light receptor 

Fusion protein of light responsive domains of CcaS from Synechocystis sp. PCC 6803 and histidine kinase EnvZ.
For the use as a green light activated actuator in E. coli.


Usage and Biology

  GlrN is the fusion protein of light responsive domains of CcaS and EnvZ designed by changing the histidine kinase region of CcaS to EnvZ domain of cph8 (BBa_I15010).

  * Requires phycocyanobilin biosynthetic genes (BBa_I15008 and BBa_I15009) for chromophore formation. 
  ** Must be used in E. coli deficient in natural EnvZ.


Expecting function (without experimental proof)

  Exposure to green light (535 nm) phosphorylate EnvZ histidin kinase domain. The activated EnvZ phosphorylates endogenous OmpR, a transcription factor which activates transcription from the OmpR positive promoter (BBa_R0083).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 606
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 860
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None