Primer

Part:BBa_K4712062

Designed by: Ke Zhang   Group: iGEM23_SMS-Shenzhen   (2023-09-29)
Revision as of 12:23, 12 October 2023 by Ke-666 (Talk | contribs)


NME-F4

The primers were designed using NCBI BLAST and SanpGene to achieve efficient and specific amplification. This primer in RPA serves as the initial binding point for the amplification process, ensuring the specificity of the reaction by targeting the desired Neisseria meningitidis DNA or RNA sequences. The primers provided data for mathematical modeling for further primer design.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


ReagentStock ConcentrationVolume Added(μL)
Forward Primer10μM1
Reverse Primer10μM1
Rehydration Buffer (2X)10
DNA Template10nM/L2
ddH2OTo 18
Starter (10X)2

fig1c.png

Electrophoresis of RPA Primer Screening for Neisseria meningitidis (NME)

fig3b.png

The figure above correspond to the fluorescence intensity of crRNA targeting Neisseria meningitidis after CRISPR reaction under Bright and UV illumination. The figure utilize pseudocolor to facilitate analysis using software (Image Lab 6). No crRNA is added into negative control. The concentration of DNA template is 10nM/L.

fig3a.png

The linear graphs sequentially correspond to the efficiency verification of crRNAs targeting the DNA sequences of Neisseria meningitidis pathogen. No crRNA is added into negative control. The concentration of DNA template is 10nM/L.

fig10c-left.png fig10c-right.png

Linear graphs and figure correspond to the fluorescence intensity of crRNAs targeting NME after one-tube reaction of RPA and CRISPR under Bright and UV illumination. The figures utilize pseudocolor to facilitate analysis using software (Image Lab 6). No crRNA is added into negative control. The concentration of DNA template is 10nM/L.

fig1.png

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Parameters
None