![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K4785012
Designed by: Junfei Xia Group: iGEM23_SUSTech-CHINA (2023-10-11)
pTetR-LasR-luxR-HMGB1-luxR-pslG-luxR-lysis_E7
The tetR promoter constitutively enhances expression of transcriptional factor LasR, which can bind to AHL 3OC12HSL. Then, LasR-3OC12HSL activator complex can open luxR promoter, leading to production target proteins(HMGB1 and pslG) and lysisE7. LysisE7 can be produced to lyse bacterial cell body and release target proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 410
Illegal AgeI site found at 607 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1020
Illegal BsaI.rc site found at 1755
Illegal BsaI.rc site found at 3081
Illegal SapI.rc site found at 1177
[edit]
Categories
Parameters
None |