Coding

Part:BBa_K4825017:Design

Designed by: Ye Siyi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 08:26, 12 October 2023 by NiWeizhao (Talk | contribs)


ATF1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 412
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 412
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 412
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 412
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 412
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


Design Notes

1. A strong promoter pTDH3 was used to maximize the conversion from isopentanol to isoamyl acetate.


Source

Saccharomyces cerevisiae

References

Verstrepen KJ, Van Laere SD, Vanderhaegen BM, Derdelinckx G, Dufour JP, Pretorius IS, Winderickx J, Thevelein JM, Delvaux FR. Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-ATF1, and ATF2 control the formation of a broad range of volatile esters. Appl Environ Microbiol. 2003 Sep;69(9):5228-37. doi: 10.1128/AEM.69.9.5228-5237.2003. PMID: 12957907; PMCID: PMC194970.