Coding

Part:BBa_K4825006:Design

Designed by: Cai Luxi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 08:10, 12 October 2023 by NiWeizhao (Talk | contribs)


OPT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 268
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 268
    Illegal XhoI site found at 244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A9D and A20T mutations are made from the signal peptide ScMα to improve the production of heterologous proteins in S.cerevisiae as a universal signal peptide.


Source

Saccharomyces cerevisiae

References

Aza P, Molpeceres G, de Salas F, Camarero S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci. 2021 Apr;78(7):3691-3707. doi: 10.1007/s00018-021-03793-y. Epub 2021 Mar 9. PMID: 33687500; PMCID: PMC8038962.