Device

Part:BBa_K283023:Design

Designed by: Liu Chenli   Group: iGEM09_HKU-HKBU   (2009-10-11)
Revision as of 19:40, 20 October 2009 by NelsonChu (Talk | contribs) (→‎Design Notes)

plux-rbs-CI-terminator-plambda(R-O12)-rbs-cheZ-terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal NheI site found at 2472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal BglII site found at 332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

composited from R0062, B0034, K094011, K094013, K094002 and K094100 The origin of the cheZ gene comes from E. Coli strain MG1655 chromosome.

References