Coding

Part:BBa_K5002345

Designed by: Haoran Yang   Group: iGEM23_Squirrel-Beijing-I   (2023-10-12)
Revision as of 11:28, 12 October 2023 by Registry (Talk | contribs)

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a cellulose synthesis operon+plant cellulose discomposing genes

The discharge of printing and dyeing wastewater has a very bad impact on the environment, we wanted to reduce the content of harmful substances in printing and dyeing wastewater to the standard of discharge. Therefore, we decided construct a cellulose synthesis operon and a group of plant cellulose discomposing genes. Cellulose synthase (acsAB) is composed of two domains: acsAB is the catalytic domain, and the cyclic di-GMP binding domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 185
    Illegal BglII site found at 1764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 799
    Illegal NgoMIV site found at 1803
    Illegal NgoMIV site found at 1872
    Illegal NgoMIV site found at 1891
    Illegal AgeI site found at 1750
    Illegal AgeI site found at 2383
    Illegal AgeI site found at 3382
    Illegal AgeI site found at 4231
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1369
    Illegal BsaI.rc site found at 2617
    Illegal SapI.rc site found at 439


[edit]
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Parameters
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