Reporter

Part:BBa_K182101

Designed by: Zuzana Hruskova and Calum de Burgh   Group: iGEM09_Aberdeen_Scotland   (2009-07-07)
Revision as of 03:36, 23 September 2009 by Calum (Talk | contribs)

pLux-Lac Hybrid with GFP Reporter and Double Terminator

Hybrid Promoter with LacO site and pLux-Lac sites for Quorum Sensing reporting via GFP.

As it also responds to LacI, this part acts as an AND logic gate, relying upon the inputs of AHL and IPTG (or some other form of LacI repression) in order to produce the GFP output

Comprised of parts BBa_I751502 (as developed by Tokyo Tech) upstream and BBa_E0840 downstream in a pSB3T5 vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 747


[edit]
Categories
Parameters
None