Device
Part:BBa_K176220
Designed by: Danqian Liu, Zongxiao He, Hao Jiang Group: iGEM09_USTC (2009-10-08)
cell density->GFP: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFP
GFP = (Cell Density * pCon 0.04) + Input AHL
High level GFP inhibits cell growth.
This part is both a cell density reporter and a cell density controller.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 856
Illegal NheI site found at 879 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 698
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1834
Illegal BsaI.rc site found at 2578
[edit]
Categories
Parameters
None |