Protein_Domain
DigA

Part:BBa_K243003

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-12)
Revision as of 17:17, 19 October 2009 by Timo (Talk | contribs) (→‎Usage and Biology)

Digoxigenin binding protein (DigA)
This part functions as a linker to another part/protein ,which is labeled with digoxigenin.


Usage and Biology

Normally a digoxigenin antibody is use for Nonradioactive in situ hybridization.

overview of immunohistochemical applications

We used this part to connect our universal restriction endonuclease protein domains to digoxigenin labeled oligos. These oligos were hybridized with ssDNA and so the made a connection between the protein and the DNA. Another possiblity is to use TFO's(triplex-forming oligonucleotides)labeled with digoxigenin, to made a connection between dsDNA and protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/receptor/antibody
Parameters
n/aDigoxigenin binding protein (DigA)