Composite

Part:BBa_K243017:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 18:06, 20 October 2009 by Timo (Talk | contribs) (→‎Design Notes)

Strep-DigA-Split Linker-Fok_a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1132


Design Notes

The choice of the linker, lipocalin tag and purification tag allows us several different combinations. Commented GenBank file

Source

Combined the parts by serial cloning steps.

References