Coding

Part:BBa_K4990011

Designed by: Dingjian Zhang   Group: iGEM23_CPU-CHINA   (2023-10-11)
Revision as of 10:24, 12 October 2023 by Norreland (Talk | contribs) (Usage in short)


Bacteria-Targeting Rod

Usage in short

You can use it to achieve BL-Fn adhesion.

You need to know

Acurate targeting is one important characteristics for many emerging cancer therapies, that is also what ourproject’s ambition. We designed two fusion proteins, they function like fishingrod, so we gave them the name: Fishing Rod Proteins (FRPs). The one that targetFn names Bacteria Tageting Rod(BTR), the other that target CRC cells names Tumor Tageting Rod(TTR). FRPs are displayed on engineered BL membranes, helping BLtarget to the correct place. Of course, their targets are cancer cells and Fn,rather than fish. Each FRP consists of four parts (Figure1): the signal peptide(white),helping FRPs locate on cell membrane; the membrane protein(pink), the base ofFRPs; and the linker(blue), helping FRPs become flexible; and the targeting fragments(yellow), the most pivotal role in FRPs

What is it

It consists of the GL-BP membrane protein, three repeated G4S linker peptides, and HlpA. Essentially, it involves the surface display technique to express HlpA on the BL membrane for adhesion to HSPG, thereby achieving adhesion between BL and CRC.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 358
    Illegal PstI site found at 1450
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 358
    Illegal PstI site found at 1450
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 771
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 358
    Illegal PstI site found at 1450
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 358
    Illegal PstI site found at 1450
    Illegal NgoMIV site found at 1050
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None