Coding

Part:BBa_K4579028:Design

Designed by: Alexa Morton   Group: iGEM23_Austin-UTexas   (2023-10-04)
Revision as of 09:12, 4 October 2023 by Registry (Talk | contribs)

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LuxR activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 904
    Illegal XhoI site found at 939
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

[design], we were unable to successfully clone this part into a part plasmid. The closest we came to achieving such a success was a plasmid that contained one correct copy of luxR and one heavily mutated copy. However, despite three attempts at sequencing, the part was never entirely correct. Because we have 6 other inducible promoter-regulator systems that have cloned into part plasmids successfully, we have not attempted any further cloning of luxR at this time.


Source

[source]

References